2024 Chlorogenic assay

Chlorogenic assay

Author: cnbp

August undefined, 2024

WebJan 1, 2024 · Chlorogenic acid showed a mixed-type inhibitory action on α-amylase. ... (PPA) were analysed by an inhibition assay, fluorescence quenching and circular dichroism (CD) measurements. Furthermore, the potential molecular interaction between CHA and PPA was evaluated by molecular docking. By providing a further understanding of the … A clonogenic assay is a cell biology technique for studying the effectiveness of specific agents on the survival and proliferation of cells. It is frequently used in cancer research laboratories to determine the effect of drugs or radiation on proliferating tumor cells as well as for titration of Cell-killing Particles (CKPs) in virus stocks. It was first developed by T.T. Puck and Philip I. Marcus at the University of Colorado in 1955.

Chromogenic Factor VIII and IX Assays: Impact on Diagnosis

WebMethods and results: In the present work, the intestinal absorption of chlorogenic acid and quinic acid, one of its most prominent intestinal biotransformation products, was studied by an in vitro permeability assay using a human Caco-2 cell line model. For both chlorogenic acid and quinic acid, the involvement of an active efflux mechanism was ... WebMar 29, 2024 · Chlorogenic acid was the major component in all the tested pear cultivars. The highest amount of chlorogenic acid ( mg/g) was found in pear samples of the cultivar “Grabova,” and the highest amount of flavonol compounds ( mg/g) was found in pear samples of the cultivar “Concordia.” 1. Introduction state street station milwaukee

Chlorogenic acid composition and method of weight …

WebChlorogenic acid (CGA), also known as 5-O-caffeoylquinic acid, is a polyphenol commonly found in human dietary products, especially in coffee beans. Polyphenols act as curative … WebAug 1, 2012 · Chlorogenic acid (CGA) is a plant polyphenol with known antioxidant properties. Although some studies suggest that CGA has anticancer properties, others indicate that this dietary constituent may cause DNA damage and induce carcinogenic effects. ... (the comet assay and the γ- H2AX focus assay) and observed that CGA (0.5 … WebMay 1, 2004 · Chlorogenic acids showed the lowest response to this colorimetric assay. The intensity of color is dependent on the formation of molybdotungstophosphate blue, which resulted from the oxidation of the phenols by a yellow molybdotungstophosphoric heteropolyanion reagent ( Slinkard & Singleton, 1977 ). state street station apartments wauwatosa

Chlorogenic acids, caffeine content and antioxidant

Determination of Total Chlorogenic Acids in Commercial …

WebThe aim of this work was to study the chemical composition, antioxidant and antibacterial properties of Achillea distans Waldst. et Kit. subsp. distans and Achillea distans Waldst. et Kit. subsp. alpina Rochel, from the Rodna Mountains (Romania). The identification and quantification of major phenolic compounds was performed by a HPLC-MS method. The … WebJan 17, 2011 · Antioxidant activities of caffeic acid and chlorogenic acid determined by the TAP assay. Each value represents the mean with S.D. of three independent experiments. Chlorogenic acid is hydrolyzed to caffeic acid in the small intestine, and it is known to be poorly absorbed ( Dupas et al., 2006 ). state street storage shelley idaho state street summer internship 2023

"WebJan 29, 2024 · The leaves of Lannea coromandelica (Houtt.) Merr. are used in the Garo, Pahan, and Teli tribal communities of Bangladesh as a traditional medicinal plant to treat hepatitis, diabetes, ulcers, heart disease, and dysentery. However, there have been limited phytochemical and biological studies on the bark of L. coromandelica. This study aimed … " - Chlorogenic assay

Chlorogenic assay

Protective Effects of Silymarin, Alone or in Combination with ...

WebMar 8, 2024 · Chlorogenic acid can be used to lower blood pressure. It is a natural compound found in many foods, including coffee, tea, fruits, and vegetables. … WebBoth chlorogenic acids (CGAs), which are the purported bioactives, and caffeine were measured using 5-chloroquinic acid as the standard and published extinction …

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WebDiscovering new natural resources of polyphenols is the aim of many recent studies in the field of natural product research. This study tentatively investigated the polyphenols profile of the stems of seven Mammillaria species (M. rhodantha, M. spinosissima, M. hahniana, M. crucigera, M. candida, M. albilanata, and M. muehlenpfordtii) using high performance … WebChlorogenic, caffeic, ferulic, and p-coumaric acids, isoquercitrin, and coumarin were identified as the major compounds in the optimised extract. The optimised extract presented anti-hemolytic and anti-hypertensive activity in vitro, in addition to showing stability and reversibility of anthocyanins and antioxidant activity with pH variation.

WebThe results of the alkaline comet assay revealed that quinic acid and chlorogenic acid decreased the DNA damage induced by X-ray irradiation and provided a significant radioprotective effect. Quinic acid decreased the presence of irradiation-induced DNA damage by 5.99-53.57% and chlorogenic acid by 4.49-48.15%, as determined by the … WebA composition formulated from sunflower, including Helianthus annulus and methods for use of the composition for treating obesity in a subject.

WebMoreover, MTT assay demonstrated chlorogenic acid could enhance proliferation of RLE-6TN cells induced by TGFβ1 in vitro. And the apoptosis assays indicated that chlorogenic acid could significantly inhibit cell apoptosis both in vivo and in vitro studies. Chlorogenic acid could inhibit the pulmonary fibrosis through endoplasmic reticulum ... WebMar 1, 2001 · The interassay CV of the folate assay was <12% and that of the vitamin B-12 assay was <7%. For the measurement of vitamin B-6 in the chlorogenic acid and placebo periods, we also collected fasting blood samples into vacuum tubes containing lithium-heparin and immediately stored the tubes at −80°C until analysis.

WebJul 1, 2024 · Cydonia oblonga miller (quince) plant serves as a potential folk medicine for treating hypertension and cardiovascular diseases in China. However, to the best of our knowledge, no study has been conducted on the polyphenolic profile and anti-adipogenic effect of quince fruit grown in China. In the current study, we aimed to investigate the …

WebMar 28, 2024 · In the chromogenic factor VIII assay, also known as the two-stage assay, the main rate-limiting step is the factor VIII level present in the test plasma. In stage one, … state street station tosaWebMay 1, 2004 · Chlorogenic acids showed the lowest response to this colorimetric assay. The intensity of color is dependent on the formation of molybdotungstophosphate blue, … state street target retirement 2020 class ivWebFeb 9, 2016 · Chlorogenic acids and caffeine are important for flavor formation as well as the health effect of green coffee brews and its extracts. The content of these … state street summer internship londonWebApr 25, 2024 · 1. Introduction. Chlorogenic acid (CGA), an ester of the caffeic and quinic acids, is often isolated from natural plants such as coffee, pears, plums, potatoes, tomatoes, carrots, and oilseeds (Liang & Kitts, 2016).Like other dietary polyphenols, CGA shows pharmacological and nutritional properties, such as antitumor influences (de Oliveira … state street syntel services pvt. ltdWebChlorogenic acid (CGA) is one of the polyphenols identified in the human diet. Previous studies have shown that CGA plays a protective role against liver diseases. … state street theater ann arborWebAug 1, 2010 · An LC–MS/MS method for the simultaneous determination of chlorogenic acid, forsythiaside A and baicalin in rat plasma and its application to pharmacokinetic study of Shuang-huang-lian in rats - ScienceDirect Journal of Pharmaceutical and Biomedical Analysis Volume 52, Issue 4, 1 August 2010, Pages 625-630 Short communication state street the layoffWebNov 1, 2001 · A class of chlorogenic acid derivatives, which potently and specifically inhibit G6Pase activity by blocking the translocase of the G6Pase complex, has recently been developed and eventually will be aimed at treating hyperglycemic conditions in type 2 diabetes (20,21,22).Infusion of members of this class of compounds in anesthetized rats … state street theater co