WebDec 14, 2009 · Neutral lipid droplets and phospholipidosis were analyzed using Invitrogen's HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit for image-based high-content screening assays. Staining … WebDec 2, 2024 · Adipocyte lipid droplet staining was performed with HCS LipidTOX™ Deep Red Neutral Lipid Stain (Thermo Fisher, Waltham, MA, USA). After washing cells with PBS three times, the adipocytes were fixed with 4% formaldehyde and incubated for 30 min at room temperature.

Tools to Measure Cell Health and Cytotoxicity Using High

WebSep 11, 2013 · For neutral lipid staining, lipid droplets were incubated for 10 min in HCS LipidTOX Neutral Red Lipid Stain (Molecular Probes) as recommended by the supplier. A Zeiss Axio Imager microscope with fluorescence and Nomarski optics and a Roper CoolSnap HQ2 camera coupled to a Zeiss AxioVision driver was used. WebLipidTox stains live cells nicely, and the lipid droplets are stable for a few days as long as the cells are healthy. There will be basal uptake of the stain into non-lipid droplet containing ... cd 郵送 方法 封筒

Invitrogen HCS LipidTOX Deep Red Neutral Lipid Stain, for cellular

WebJan 1, 2014 · HCS LipidTOX Red staining 1. LipidTOX Red staining solution: Dilute HCS LipidTOX Red (Invitrogen) 1:200 in PBS. Prepare 40 μl per cover slip; allow 10% extra for pipetting errors. 2. 0.2% Triton X-100 in PBS (v/v). 3. Incubation chamber: Prepare a dark chamber big enough to store all cover slips. WebDec 27, 2024 · Cells were stained with 0.5 μM NIM-7 for 1 h, or HCS LipidTOX™ Deep Red Neutral Lipid Stain (1 : 1000 dilution) for 0.5 h, or 50 nM LysoTracker® Blue DND-22 for 0.5 h, or 50 nM MitoTracker™ … WebApr 12, 2024 · To quantify the number adipocytes, we used HCS LipidTOX™ Deep Red neutral lipid stain (H34477, Thermofisher). The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid ... cd 題名 印刷