Lambda pir gene
Tīmeklis2024. gada 1. jūn. · S17-1 lambda pir Strain constructed that harbors the lambda pir gene de Lorenzo et al., 1993 Plasmids pUC57- ermC pUC57 vector harboring the Erm § gene Ji et al., 2024 Tīmeklis2009. gada 30. jūl. · They should be readily available from a number of commercial vendors. pGP704 is lambda pir-dependent, it does not carry the lambda pir gene. If …
Lambda pir gene
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TīmeklisS17-1 λpir strain contains the pir gene (λpir). This strain have chromosomally integrated conjugal transfer functions (RP4 transfer functions), so when it is used as specific … TīmeklisE. coli SM10 λpir strain contains the pir gene (lysogenized with λpir phage) and it is designed for cloning and propagation of plasmids with R6K origin of replication sequence (such as pUT mini-Tn5 vectors). The plasmids containing the R6K origin of replication require the R6K-spicific replication protein lpir gene for propagation. …
TīmeklisPIR protein expression summary. This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein express ed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3 … TīmeklisAllelic exchange is frequently used in bacteria to generate knockout mutants in genes of interest, to carry out phenotypic analysis and learn about their function. Frequently, understanding of gene function in complex processes such as pathogenesis requires the generation of multiple mutant strains. …
Tīmeklis2024. gada 15. marts · Immunoglobulin lambda constant 2 2 publications. Alternative names. ... Ig lambda chain C region X 1 publication. Ig lambda-2 chain C region Curated. Gene names. Name. IGLC2 2 publications. Organism names. Organism. Homo sapiens (Human) Taxonomic identifier. ... PIR. A92057 L2HU; Sequence Protein … Tīmeklis2024. gada 16. okt. · Donor strain (mobilizing strain) carrying the transfer genes of the broad host range IncP type plasmid RP4 integrated in its chromosome. Useful for …
TīmeklisGrowth in Bacteria Bacterial Resistance (s) Ampicillin and Kanamycin, 100 & 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5a lambda pir Copy number Unknown Gene/Insert Gene/Insert name FRT-kan-FRT Promoter R6K Cloning Information Cloning method Unknown 5′ sequencing primer R6K-3' 3′ sequencing primer rrnB-T1 …
Tīmeklis2024. gada 16. okt. · Name DH5αpir Synonyms DH5 alpha pir Genotype endA1 hsdR17 glnV44 (= supE44) thi-1 recA1 gyrA96 relA1 φ80dlacΔ(lacZ)M15 Δ(lacZYA … talk about preferencesTīmeklis1991. gada 20. dec. · First, E. coli SM10 lambda pir (pKNG105) was mated with strains of Y. enterocolitica. This led to the integration of pKNG105 into the chromosome, by … two eared listener meaningTīmeklis2024. gada 15. febr. · Chromosomal genes carried in the F plasmid are listed in brackets. ΔH1= removes part of cro and all genes to the right of it rB/K+/-= The (B/K) defines the strain lineage. The +/- indicates whether the strain has or hasn't got the restriction system. mB/K+/-= The (B/K) defines the strain lineage. talk about phone numberTīmeklisBuy Escherichia coli; Strain S17-1 Lambda pir reference standards from Microorganisms. Available to purchase online at LGC Standards. We use cookies to … two eared catTīmeklisAbstract. Phage lambda gene Q encodes a positive regulator of phage late gene expression. We show that purified Q gene protein functions as a transcription … talk about radios with headsets for motorolaTīmeklispKD3 and pKD4 are identical except for the region between the FRT sites, so they create an identical scar that has stop codons in all six reading frames. As drawn in … talk about rain radio lyricsTīmeklis2024. gada 24. maijs · Outline of the λ-Red assisted homology-dependent recombination for large synthetic pathways integration in Escherichia coli.a Construction of plasmid pRC-IS5 with large synthetic pathways. pRC-IS5 (including R6K and a homologous region) replicates normally in E. coli with the expression of pir + protein … talkaboutshirts